| Product Name |
Anti His-Tag Mouse Monoclonal Antibody, HRP conjugated |
| Aliases |
6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag |
| Background |
The His-Tag (or Polyhistidine tag) is a short amino acid sequence, typically composed of six to ten consecutive histidine residues (e.g., His6), genetically engineered onto either the N- or C-terminus of a recombinant protein. Its primary function is to facilitate the purification of the tagged protein using immobilized metal affinity chromatography (IMAC). Histidine residues possess an imidazole side chain that exhibits high affinity for certain divalent metal ions, such as nickel (Ni2+), cobalt (Co2+), copper (Cu2+), or zinc (Zn2+). This specific interaction allows the His-tagged protein to bind tightly to a chromatography resin charged with one of these metal ions. |
| Application Information |
Molecular Weight: Recombinant protein dependent
Clonality: Mouse monoclonal antibody
Species Reactivity: Recombinant protein
Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Conjugation: HRP
|
| Immunogen |
A synthesized peptide derived from His-tag |
| Storage Buffer |
Supplied in PBS (pH7.2) containing 50% glycerol, 1% BSA and 0.02% sodium azide. |
| Storage |
Store at -20 °C for one year. |
| Recommended Dilutions |
Western Blotting (WB): 1:2,000-1:5,000
Immunocytochemistry (IC): 1:500-1:2,000 |
| Note |
This product is for research use only. |
