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KD-Validated Anti-Caspase 9 Rabbit Monoclonal Antibody #63154

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Flow cytometric analysis of caspase 9 expression in HepG2 cells using anti-caspase 9 antibody (Cat#63154, 1:2,000). Green, isotype control; red, caspase 9.

Western blotting analysis using anti-caspase 9 antibody (Cat#63154). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 9 antibody (Cat#63154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-caspase 9 antibody (Cat#63154). Caspase 9 expression in wild type (WT) and caspase 9 (CASP9) shRNA knockdown (KD) HeLa cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-caspase 9 antibody (Cat#63154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CASP9
货号：

63154
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-Caspase 9 Rabbit Monoclonal Antibody
Aliases	Caspase 9; ICE-LAP6; APAF-3; MCH6; PPP1R56; Caspase 9, Apoptosis-Related Cysteine Peptidase; Protein Phosphatase 1, Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; Caspase-9; Caspase 9, Apoptosis-Related Cysteine Protease; Apoptotic Protease Activating Factor 3; Apoptotic Protease-Activating Factor 3; Apoptotic Protease MCH-6; Apoptotic Protease Mch-6; EC 3.4.22.62; CASP-9; APAF3
Background	Gene Name: CASP9 NCBI Gene Entry: 842 UniProt Entry: P55211
Application Information	Molecular Weight: Predicted, 46 kDa, observed, 46 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4275 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Caspase-9
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of caspase 9 expression in HepG2 cells using anti-caspase 9 antibody (Cat#63154, 1:2,000). Green, isotype control; red, caspase 9.
Western blotting analysis using anti-caspase 9 antibody (Cat#63154). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 9 antibody (Cat#63154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-caspase 9 antibody (Cat#63154). Caspase 9 expression in wild type (WT) and caspase 9 (CASP9) shRNA knockdown (KD) HeLa cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-caspase 9 antibody (Cat#63154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CASP9
货号：

63154
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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