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Anti-ROCK1 Rabbit Monoclonal Antibody #1163

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Flow cytometric analysis of ROCK1 expression in HepG2 cells using ROCK1 antibody (Cat#1163, 1:2,000). Green, isotype control; red, ROCK1.

Immunocytochemical staining of HepG2 cells with ROCK1 antibody (Cat#1163, 1:1,000). Nuclei were stained blue with DAPI; ROCK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-ROCK1 antibody (Cat#1163). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ROCK1 antibody (Cat#1163, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ROCK1, Rho associated coiled-coil containing protein kinase 1.

基因名：

ROCK1
货号：

1163
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-ROCK1 Rabbit Monoclonal Antibody
Aliases	ROCK1; Rho Associated Coiled-Coil Containing Protein Kinase 1; P160ROCK; Renal Carcinoma Antigen NY-REN-35; Rho-Associated Protein Kinase 1; P160 ROCK-1; EC 2.7.11.1; ROCK-I; Rho-Associated, Coiled-Coil-Containing Protein Kinase 1; Rho-Associated, Coiled-Coil-Containing Protein Kinase I; EC 2.7.11
Background	Gene Name: ROCK1 NCBI Gene Entry: 6093 UniProt Entry: Q13464
Application Information	Molecular Weight: Predicted, 158 kDa; observed, 158 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2065 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human ROCK1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ROCK1 expression in HepG2 cells using ROCK1 antibody (Cat#1163, 1:2,000). Green, isotype control; red, ROCK1.
Immunocytochemical staining of HepG2 cells with ROCK1 antibody (Cat#1163, 1:1,000). Nuclei were stained blue with DAPI; ROCK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ROCK1 antibody (Cat#1163). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ROCK1 antibody (Cat#1163, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ROCK1, Rho associated coiled-coil containing protein kinase 1.

Citation(0)

基因名：

ROCK1
货号：

1163
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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