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Anti-NFKB Inhibitor Beta Rabbit Monoclonal Antibody #2306

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Flow cytometric analysis of IKB beta expression in HepG2 cells using IKB beta antibody (Cat#2306, 1:2,000). Green, isotype control; red, IKB beta.
Immunocytochemical staining of HepG2 cells with anti-IKB beta antibody (Cat#2306, 1:1,000). Nuclei were stained blue with DAPI; IKB beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-IKB beta antibody (Cat#2306). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IKB beta antibody (Cat#2306, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    NFKBIB
  • 货号:
    2306
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-NFKB Inhibitor Beta Rabbit Monoclonal Antibody
Aliases NFKB Inhibitor Beta; TRIP9; IKBB; NF-Kappa-B Inhibitor Beta; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Beta; Thyroid Receptor-Interacting Protein 9; TR-Interacting Protein 9; I-Kappa-B-Beta; NF-Kappa-BIB; IkappaBbeta; IkB-Beta; TRIP-9; IkB-B
Background

Gene Name: NFKBIB

NCBI Gene Entry: 4793

UniProt Entry: Q15653

Application Information

Molecular Weight: Predicted, 38 kDa; observed, 48 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB235

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human IKB beta
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of IKB beta expression in HepG2 cells using IKB beta antibody (Cat#2306, 1:2,000). Green, isotype control; red, IKB beta.
  • Immunocytochemical staining of HepG2 cells with anti-IKB beta antibody (Cat#2306, 1:1,000). Nuclei were stained blue with DAPI; IKB beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-IKB beta antibody (Cat#2306). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IKB beta antibody (Cat#2306, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of IKB beta expression in HepG2 cells using IKB beta antibody (Cat#2306, 1:2,000). Green, isotype control; red, IKB beta.
Immunocytochemical staining of HepG2 cells with anti-IKB beta antibody (Cat#2306, 1:1,000). Nuclei were stained blue with DAPI; IKB beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-IKB beta antibody (Cat#2306). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IKB beta antibody (Cat#2306, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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