Anti-POU Class 5 Homeobox 1 Rabbit Monoclonal Antibody - 产品中心

Anti-POU Class 5 Homeobox 1 Rabbit Monoclonal Antibody #2565

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Flow cytometric analysis of POU5F1 expression in HAP-1 cells using anti-POU5F1 antibody (Cat#2565, 1:2,000). Green, isotype control; red, POU5F1.

Immunocytochemical staining of HAP1 cells with anti-POU5F1 antibody (Cat#2565, 1:1,000) . Nuclei were stained blue with DAPI;POU5F1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-POU5F1 antibody (Cat#2565). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-POU5F1 antibody (Cat#2565, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

POU5F1
货号：

2565
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-POU Class 5 Homeobox 1 Rabbit Monoclonal Antibody
Aliases	POU5F1; POU Class 5 Homeobox 1; OCT3; Oct3/4; OCT-4; Oct4; OTF3; POU Domain, Class 5, Transcription Factor 1; Octamer-Binding Transcription Factor 3; Octamer-Binding Protein 3; Octamer-Binding Protein 4; MGC22487; OTF-3; Oct-3; POU-Type Homeodomain-Containing DNA-Binding Protein; POU Domain Class 5, Transcription Factor 1; POU Domain Transcription Factor OCT4; Oct-4; OTF4; OCT4
Background	Gene Name: POU5F1 NCBI Gene Entry: 5460 UniProt Entry: Q01860
Application Information	Molecular Weight: Predicted, 39 kDa; observed, 45 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1425 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Oct4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of POU5F1 expression in HAP-1 cells using anti-POU5F1 antibody (Cat#2565, 1:2,000). Green, isotype control; red, POU5F1.
Immunocytochemical staining of HAP1 cells with anti-POU5F1 antibody (Cat#2565, 1:1,000) . Nuclei were stained blue with DAPI;POU5F1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-POU5F1 antibody (Cat#2565). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-POU5F1 antibody (Cat#2565, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

POU5F1
货号：

2565
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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