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Anti-Lysine Demethylase 1 Rabbit Monoclonal Antibody #2928

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Flow cytometric analysis of Lysine demethylase 1A expression in HepG2 cells using anti-Lysine demethylase 1A antibody (Cat#2928, 1:2,000). Green, isotype control; red, Lysine demethylase 1A.
Immunocytochemical staining of HepG2 cells with anti-Lysine demethylase 1A antibody (Cat#2928, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Lysine demethylase 1A antibody (Cat#2928). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lysine demethylase 1A antibody (Cat#2928, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    KDM1A
  • 货号:
    2928
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Lysine Demethylase 1 Rabbit Monoclonal Antibody
Aliases KDM1A; Lysine Demethylase 1; LSD; Lysine-Specific Histone Demethylase 1A; KIAA0601; BHC110; AOF2; KDM1; [Histone H3]-Dimethyl-L-Lysine(4) FAD-Dependent Demethylase 1A; Flavin-Containing Amine Oxidase Domain-Containing Protein 2; Amine Oxidase (Flavin Containing) Domain 2; BRAF35-HDAC Complex Protein BHC110; FAD-Binding Protein BRAF35-HDAC Complex, 110 KDa Subunit; Lysine-Specific Histone Demethylase 1; Lysine (K)-Specific Demethylase 1A; Lysine (K)-Specific Demethylase 1; EC 1.14.99.66; CPRF
Background

Gene Name: KDM1A

NCBI Gene Entry: 23028

UniProt Entry: O60341

Application Information

Molecular Weight: Predicted, 93 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3215

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human KDM1 / LSD1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Lysine demethylase 1A expression in HepG2 cells using anti-Lysine demethylase 1A antibody (Cat#2928, 1:2,000). Green, isotype control; red, Lysine demethylase 1A.
  • Immunocytochemical staining of HepG2 cells with anti-Lysine demethylase 1A antibody (Cat#2928, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Lysine demethylase 1A antibody (Cat#2928). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lysine demethylase 1A antibody (Cat#2928, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Lysine demethylase 1A expression in HepG2 cells using anti-Lysine demethylase 1A antibody (Cat#2928, 1:2,000). Green, isotype control; red, Lysine demethylase 1A.
Immunocytochemical staining of HepG2 cells with anti-Lysine demethylase 1A antibody (Cat#2928, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Lysine demethylase 1A antibody (Cat#2928). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lysine demethylase 1A antibody (Cat#2928, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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