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Anti-SYNE1 Rabbit Monoclonal Antibody #3008

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Flow cytometric analysis of SYNE1 expression in HepG2 cells using anti-SYNE1 antibody (Cat#3008, 1:2,000). Green, isotype control; red, SYNE1.
Immunocytochemical staining of HepG2 cells with anti-SYNE1 antibody (Cat#3008, 1:1,000). Nuclei were stained blue with DAPI; SYNE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SYNE1 antibody (Cat#3008). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SYNE1 antibody (Cat#3008, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    SYNE1
  • 货号:
    3008
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-SYNE1 Rabbit Monoclonal Antibody
Aliases SYNE1; Spectrin Repeat Containing Nuclear Envelope Protein 1; Nesprin-1; Enaptin; MYNE1; Myocyte Nuclear Envelope Protein 1; KIAA0796; DJ45H2.2; C6orf98; SCAR8; ARCA1; Nesp1; CPG2; 8B; Spectrin Repeat Containing, Nuclear Envelope 1; Synaptic Nuclear Envelope Protein 1; KASH Domain-Containing Protein 1; SYNE-1B; KASH1; Nuclear Envelope Spectrin Repeat Protein 1; Nuclear Envelope Spectrin Repeat-1; Chromosome 6 Open Reading Frame 98; Synaptic Nuclei Expressed Gene 1; CPG2 Full Length; EC 4.2.1.49; EC 4.3.1.3; Nesprin 1; KIAA1262; KIAA1756; Myne-1; Syne-1; EDMD4; AMC3; AMCM
Background

Gene Name: SYNE1

NCBI Gene Entry: 23345

UniProt Entry: Q8NF91

Application Information

Molecular Weight: Predicted, 112 kDa; observed, 140 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3605

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Nesprin 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of SYNE1 expression in HepG2 cells using anti-SYNE1 antibody (Cat#3008, 1:2,000). Green, isotype control; red, SYNE1.
  • Immunocytochemical staining of HepG2 cells with anti-SYNE1 antibody (Cat#3008, 1:1,000). Nuclei were stained blue with DAPI; SYNE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-SYNE1 antibody (Cat#3008). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SYNE1 antibody (Cat#3008, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of SYNE1 expression in HepG2 cells using anti-SYNE1 antibody (Cat#3008, 1:2,000). Green, isotype control; red, SYNE1.
Immunocytochemical staining of HepG2 cells with anti-SYNE1 antibody (Cat#3008, 1:1,000). Nuclei were stained blue with DAPI; SYNE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SYNE1 antibody (Cat#3008). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SYNE1 antibody (Cat#3008, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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