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Anti-Annexin A3 Mouse Monoclonal Antibody #3515

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Flow cytometric analysis of Annexin A3 expression in HepG2 cells using anti-Annexin A3 antibody (Cat#3515, 1:2,000). Green, isotype control; red, Annexin A3.

Immunocytochemical staining of HepG2 cells with anti-Annexin A3 antibody (Cat#3515, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-annexin A3 antibody (Cat#3515). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A3 antibody (Cat#3515, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ANXA3
货号：

3515
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Annexin A3 Mouse Monoclonal Antibody
Aliases	ANXA3; Annexin A3; ANX3; Inositol 1,2-Cyclic Phosphate 2-Phosphohydrolase; Placental Anticoagulant Protein III; 35-Alpha Calcimedin; Lipocortin III; Annexin-3; PAP-III; Annexin III (Lipocortin III, 1,2-Cyclic-Inositol-Phosphate Phosphodiesterase, Placental Anticoagulant Protein III, Calcimedin 35-Alpha); Annexin III (Lipocortin III); Calcimedin 35-Alpha; Annexin III
Background	Gene Name: ANXA3 NCBI Gene Entry: 306 UniProt Entry: P12429
Application Information	Molecular Weight: Predicted, 36 kDa; observed, 30 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB6300 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human ANXA3
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Annexin A3 expression in HepG2 cells using anti-Annexin A3 antibody (Cat#3515, 1:2,000). Green, isotype control; red, Annexin A3.
Immunocytochemical staining of HepG2 cells with anti-Annexin A3 antibody (Cat#3515, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-annexin A3 antibody (Cat#3515). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A3 antibody (Cat#3515, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ANXA3
货号：

3515
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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