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Anti-TBP Mouse Monoclonal Antibody #3708

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Flow cytometric analysis of TATA-box binding protein expression in HAP-1 cells using anti-TATA-box binding protein antibody (Cat#3708, 1:2,000). Green, isotype control; red, TATA-box binding protein.
Immunocytochemical staining of HAP1 cells with anti-TATA-box binding protein antibody (Cat#3708, 1:1,000) . Nuclei were stained blue with DAPI;TATA-box binding protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-TATA-box binding protein antibody (Cat#3708). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TATA-box binding protein antibody (Cat#3708, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    TBP
  • 货号：
    3708
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-TBP Mouse Monoclonal Antibody
Aliases TBP; TATA-Box Binding Protein; TFIID; GTF2D1; TBP1; Transcription Initiation Factor TFIID TBP Subunit; TATA Sequence-Binding Protein; TATA-Box-Binding Protein; TATA-Box Factor; SCA17; TATA-Box Binding Protein N-Terminal Domain; Spinocerebellar Ataxia 17; TATA-Binding Factor; GTF2D; HDL4; TF2D
Background

Gene Name: TBP

NCBI Gene Entry: 6908

UniProt Entry: P20226
Application Information

Molecular Weight: Predicted, 38 kDa; observed, 38 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7015

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human TBP
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of TATA-box binding protein expression in HAP-1 cells using anti-TATA-box binding protein antibody (Cat#3708, 1:2,000). Green, isotype control; red, TATA-box binding protein.
  • Immunocytochemical staining of HAP1 cells with anti-TATA-box binding protein antibody (Cat#3708, 1:1,000) . Nuclei were stained blue with DAPI;TATA-box binding protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-TATA-box binding protein antibody (Cat#3708). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TATA-box binding protein antibody (Cat#3708, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    TBP
  • 货号：
    3708
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of TATA-box binding protein expression in HAP-1 cells using anti-TATA-box binding protein antibody (Cat#3708, 1:2,000). Green, isotype control; red, TATA-box binding protein.
Immunocytochemical staining of HAP1 cells with anti-TATA-box binding protein antibody (Cat#3708, 1:1,000) . Nuclei were stained blue with DAPI;TATA-box binding protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-TATA-box binding protein antibody (Cat#3708). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TATA-box binding protein antibody (Cat#3708, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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