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Anti-HSPA5 Rabbit Monoclonal Antibody #4061

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Flow cytometric analysis of HSPA5 expression in HepG2 cells using anti-HSPA5 antibody (Cat#4061, 1:2,000). Green, isotype control; red, HSPA5.

Immunocytochemical staining of HepG2 cells with anti-HSPA5 antibody (Cat#4061, 1:1,000). Nuclei were stained blue with DAPI; HSPA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-HSPA5 antibody (Cat#4061). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPA5 antibody (Cat#4061, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

HSPA5
货号：

4061
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-HSPA5 Rabbit Monoclonal Antibody
Aliases	HSPA5; Heat Shock Protein Family A (Hsp70) Member 5; GRP78; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); Immunoglobulin Heavy Chain-Binding Protein; Heat Shock Protein 70 Family Protein 5; Heat Shock Protein Family A Member 5; Endoplasmic Reticulum Chaperone BiP; Glucose-Regulated Protein, 78kDa; 78 KDa Glucose-Regulated Protein; Binding-Immunoglobulin Protein; HSP70 Family Protein 5; BiP; BIP; Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD); Endoplasmic Reticulum Lumenal Ca(2+)-Binding; Protein Grp78; Epididymis Secretory Sperm Binding Protein Li 89n; EC 3.6.4.10; HEL-S-89n; GRP-78
Background	Gene Name: HSPA5 NCBI Gene Entry: 3309 UniProt Entry: P11021
Application Information	Molecular Weight: Predicted, 72 kDa; observed, 72 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB9395 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human GRP78 BiP
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of HSPA5 expression in HepG2 cells using anti-HSPA5 antibody (Cat#4061, 1:2,000). Green, isotype control; red, HSPA5.
Immunocytochemical staining of HepG2 cells with anti-HSPA5 antibody (Cat#4061, 1:1,000). Nuclei were stained blue with DAPI; HSPA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HSPA5 antibody (Cat#4061). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPA5 antibody (Cat#4061, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

HSPA5
货号：

4061
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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