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Anti-CD146 Rabbit Monoclonal Antibody #4065

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Flow cytometric analysis of CD146 expression in HepG2 cells using anti-CD146 antibody (Cat#4065, 1:2,000). Green, isotype control; red, CD146.

Immunocytochemical staining of HepG2 cells with anti-CD146 antibody (Cat#4065, 1:1,000). Nuclei were stained blue with DAPI; CD146 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-CD146 antibody (Cat#4065). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD146 antibody (Cat#4065, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

MCAM
货号：

4065
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-CD146 Rabbit Monoclonal Antibody
Aliases	MCAM; Melanoma Cell Adhesion Molecule; MUC18; METCAM; HEMCAM; CD146; S-Endo 1 Endothelial-Associated Antigen; Melanoma-Associated Antigen MUC18; Cell Surface Glycoprotein MUC18; Cell Surface Glycoprotein P1H12; Melanoma-Associated Antigen A32; Gicerin; MelCAM; Melanoma Adhesion Molecule; CD146 Antigen; MELCAM
Background	Gene Name: MCAM NCBI Gene Entry: 4162 UniProt Entry: P43121
Application Information	Molecular Weight: Predicted, 72 kDa; observed, 72 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB9415 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CD146
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CD146 expression in HepG2 cells using anti-CD146 antibody (Cat#4065, 1:2,000). Green, isotype control; red, CD146.
Immunocytochemical staining of HepG2 cells with anti-CD146 antibody (Cat#4065, 1:1,000). Nuclei were stained blue with DAPI; CD146 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CD146 antibody (Cat#4065). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD146 antibody (Cat#4065, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

MCAM
货号：

4065
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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