Anti-Golgi integral membrane protein 4 Rabbit Monoclonal Antibody - 产品中心

Anti-Golgi integral membrane protein 4 Rabbit Monoclonal Antibody #4491

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Flow cytometric analysis of golgi integral membrane protein 4 expression in HAP-1 cells using anti-golgi integral membrane protein 4 antibody (Cat#4491, 1:2,000). Green, isotype control; red, golgi integral membrane protein 4.

Immunocytochemical staining of HAP-1 cells with anti-Golgi integral membrane protein 4 antibody (Cat#4491, 1:1,000). Nuclei were stained blue with DAPI; Golgi integral membrane protein 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-golgi integral membrane protein 4 antibody (Cat#4491). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-golgi integral membrane protein 4 antibody (Cat#4491, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

GOLIM4
货号：

4491
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Golgi integral membrane protein 4 Rabbit Monoclonal Antibody
Aliases	GOLIM4; Golgi Integral Membrane Protein 4; GPP130; GIMPC; Golgi Phosphoprotein 4; GOLPH4; P138; Golgi-Localized Phosphoprotein Of 130 KDa; Golgi Integral Membrane Protein, Cis; Golgi Phosphoprotein Of 130 KDa; Cis Golgi-Localized Calcium-Binding Protein; 130 KDa Golgi-Localized Phosphoprotein; Type II Golgi Membrane Protein; GIMPc
Background	Gene Name: GOLIM4 NCBI Gene Entry: 27333 UniProt Entry: O00461
Application Information	Molecular Weight: Predicted, 82 kDa; observed, 130 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11485 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human GOLPH4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of golgi integral membrane protein 4 expression in HAP-1 cells using anti-golgi integral membrane protein 4 antibody (Cat#4491, 1:2,000). Green, isotype control; red, golgi integral membrane protein 4.
Immunocytochemical staining of HAP-1 cells with anti-Golgi integral membrane protein 4 antibody (Cat#4491, 1:1,000). Nuclei were stained blue with DAPI; Golgi integral membrane protein 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-golgi integral membrane protein 4 antibody (Cat#4491). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-golgi integral membrane protein 4 antibody (Cat#4491, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

GOLIM4
货号：

4491
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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