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Anti-BCAR1 Rabbit Monoclonal Antibody #4524

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Flow cytometric analysis of BCAR1 expression in H9C2 cells using anti-BCAR1 antibody (Cat#4524, 1:2,000). Green, isotype control; red, BCAR1.

Immunocytochemical staining of H9c2 cells with anti-BCAR1 antibody (Cat#4524, 1:1,000). Nuclei were stained blue with DAPI; BCAR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-BCAR1 antibody (Cat#4524). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCAR1 antibody (Cat#4524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

BCAR1
货号：

4524
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-BCAR1 Rabbit Monoclonal Antibody
Aliases	BCAR1; BCAR1 Scaffold Protein, Cas Family Member; CASS1; CAS; Cas Scaffolding Protein Family Member 1; CRKAS; Breast Cancer Anti-Estrogen Resistance Protein 1; BCAR1, Cas Family Scaffolding Protein; BCAR1, Cas Family Scaffold Protein; P130Cas; Breast Cancer Anti-Estrogen Resistance 1; Crk-Associated Substrate P130Cas; Crk-Associated Substrate; CRK-Associated Substrate; P130CAS; P130cas; Crkas; CAS1
Background	Gene Name: BCAR1 NCBI Gene Entry: 9564 UniProt Entry: P56945
Application Information	Molecular Weight: Predicted, 93 kDa; observed, 130 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11650 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human BCAR1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of BCAR1 expression in H9C2 cells using anti-BCAR1 antibody (Cat#4524, 1:2,000). Green, isotype control; red, BCAR1.
Immunocytochemical staining of H9c2 cells with anti-BCAR1 antibody (Cat#4524, 1:1,000). Nuclei were stained blue with DAPI; BCAR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BCAR1 antibody (Cat#4524). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCAR1 antibody (Cat#4524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

BCAR1
货号：

4524
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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