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Anti-IGF2BP1 Rabbit Monoclonal Antibody #5209

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Flow cytometric analysis of IGF2BP1 expression in HT-1080 cells using anti-IGF2BP1 antibody (Cat#5209, 1:2,000). Green, isotype control; red, IGF2BP1.

Immunocytochemical staining of HT-1080 cells with anti-IGF2BP1 antibody (Cat#5209, 1:1,000) . Nuclei were stained blue with DAPI; IGF2BP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.

Western blotting analysis using anti-IGF2BP1 antibody (Cat#5209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IGF2BP1 antibody (Cat#5209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

IGF2BP1
货号：

5209
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-IGF2BP1 Rabbit Monoclonal Antibody
Aliases	IGF2BP1; Insulin Like Growth Factor 2 MRNA Binding Protein 1; IMP-1; IMP1; Insulin-Like Growth Factor 2 MRNA-Binding Protein 1; Coding Region Determinant-Binding Protein; IGF-II MRNA-Binding Protein 1; IGF2 MRNA-Binding Protein 1; Zipcode-Binding Protein 1; VICKZ Family Member 1; CRD-BP; VICKZ1; CRDBP; ZBP-1; ZBP1; Insulin-Like Growth Factor 2 MRNA Binding Protein 1 DeltaN CRDBP; Insulin-Like Growth Factor 2 MRNA Binding Protein 1; IGF II MRNA Binding Protein 1
Background	Gene Name: IGF2BP1 NCBI Gene Entry: 10642 UniProt Entry: Q9NZI8
Application Information	Molecular Weight: Predicted, 63 kDa; observed, 65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB170 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human IGF2BP1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:1,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of IGF2BP1 expression in HT-1080 cells using anti-IGF2BP1 antibody (Cat#5209, 1:2,000). Green, isotype control; red, IGF2BP1.
Immunocytochemical staining of HT-1080 cells with anti-IGF2BP1 antibody (Cat#5209, 1:1,000) . Nuclei were stained blue with DAPI; IGF2BP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Western blotting analysis using anti-IGF2BP1 antibody (Cat#5209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IGF2BP1 antibody (Cat#5209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

IGF2BP1
货号：

5209
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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