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Anti-CD3G Rabbit Monoclonal Antibody #5444

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Western blotting analysis using anti-CD3G antibody (Cat#5444). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD3G antibody (Cat#5444, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CD3G expression in Jurkat cells using anti-CD3G antibody (Cat#5444, 1:2,000). Green, isotype control; red, CD3G.
  • 基因名:
    CD3G
  • 货号:
    5444
  • 应用:
    WB, FCM
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-CD3G Rabbit Monoclonal Antibody
Aliases CD3G; CD3 Gamma Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Gamma Chain; CD3-GAMMA; CD3GAMMA; CD3g Antigen, Gamma Polypeptide (TiT3 Complex); CD3g Molecule, Gamma (CD3-TCR Complex); T-Cell Receptor T3 Gamma Chain; T3G; T-Cell Antigen Receptor Complex, Gamma Subunit Of T3; CD3g Molecule, Epsilon (CD3-TCR Complex); CD3g Molecule; CD3g Antigen; IMD17
Background

Gene Name: CD3G

NCBI Gene Entry: 917

UniProt Entry: P09693

Application Information

Molecular Weight: Predicted, 20 kDa; observed, 18-28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1585

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human CD3G
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-CD3G antibody (Cat#5444). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD3G antibody (Cat#5444, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of CD3G expression in Jurkat cells using anti-CD3G antibody (Cat#5444, 1:2,000). Green, isotype control; red, CD3G.
Citation(0)
Western blotting analysis using anti-CD3G antibody (Cat#5444). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD3G antibody (Cat#5444, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CD3G expression in Jurkat cells using anti-CD3G antibody (Cat#5444, 1:2,000). Green, isotype control; red, CD3G.
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