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Anti-CHRNB1 Rabbit Monoclonal Antibody #5477

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Western blotting analysis using anti-CHRNB1 antibody (Cat#5477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHRNB1 antibody (Cat#5477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CHRNB1 expression in HAP-1 cells using anti-CHRNB1 antibody (Cat#5477, 1:2,000). Green, isotype control; red, CHRNB1.
  • 基因名:
    CHRNB1
  • 货号:
    5477
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-CHRNB1 Rabbit Monoclonal Antibody
Aliases CHRNB1; Cholinergic Receptor Nicotinic Beta 1 Subunit; CHRNB; Cholinergic Receptor, Nicotinic, Beta Polypeptide 1 (Muscle); Acetylcholine Receptor, Nicotinic, Beta 1 (Muscle); Cholinergic Receptor, Nicotinic, Beta 1 (Muscle); Cholinergic Receptor, Nicotinic Beta 1; Acetylcholine Receptor Subunit Beta; ACHRB; CMS1D; CMS2A; CMS2C; SCCMS
Background

Gene Name: CHRNB1

NCBI Gene Entry: 1140

UniProt Entry: P11230

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 50-57 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1670

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Acetylcholine receptor subunit beta
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-CHRNB1 antibody (Cat#5477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHRNB1 antibody (Cat#5477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of CHRNB1 expression in HAP-1 cells using anti-CHRNB1 antibody (Cat#5477, 1:2,000). Green, isotype control; red, CHRNB1.
Citation(0)
Western blotting analysis using anti-CHRNB1 antibody (Cat#5477). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHRNB1 antibody (Cat#5477, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CHRNB1 expression in HAP-1 cells using anti-CHRNB1 antibody (Cat#5477, 1:2,000). Green, isotype control; red, CHRNB1.
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