Western blotting analysis using anti-Hsp27 antibody (Cat#5525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp27 antibody (Cat#5525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
HSPB1; Heat Shock Protein Family B (Small) Member 1; HSP27; HSP28; Hs.76067; Hsp25; CMT2F; Heat Shock Protein Family B Member 1; Estrogen-Regulated 24 KDa Protein; Stress-Responsive Protein 27; Heat Shock 27kDa Protein 1; Heat Shock 27kD Protein 1; Heat Shock Protein Beta-1; 28 KDa Heat Shock Protein; Heat Shock 27 KDa Protein; SRP27; Epididymis Secretory Protein Li 102; HEL-S-102; HSP 27; HMN2B; HMND3; HspB1
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Picture
Western blotting analysis using anti-Hsp27 antibody (Cat#5525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp27 antibody (Cat#5525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Hsp27 antibody (Cat#5525). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp27 antibody (Cat#5525, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
