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KD-Validated Anti-HSPA5 Rabbit Monoclonal Antibody #61474

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Flow cytometric analysis of HSPA5 expression in HepG2 cells using HSPA5 antibody (Cat#61474, 1:2,000). Green, isotype control; red, HSPA5.
Immunocytochemical staining of HepG2 cells with anti-HSPA5 antibody (Cat#61474, 1:1,000). Nuclei were stained blue with DAPI; HSPA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-HSPA5 antibody (Cat#61474). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPA5 antibody (Cat#61474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). HSPA5, heat shock protein family A (Hsp70) member 5.
Western blotting analysis using anti-HSPA5 antibody (Cat#61474). HSPA5 expression in wild type (WT) and HSPA5 shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-HSPA5 antibody (Cat#61474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    HSPA5
  • 货号：
    61474
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-HSPA5 Rabbit Monoclonal Antibody
Aliases HSPA5; Heat Shock Protein Family A (Hsp70) Member 5; GRP78; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); Immunoglobulin Heavy Chain-Binding Protein; Heat Shock Protein 70 Family Protein 5; Heat Shock Protein Family A Member 5; Endoplasmic Reticulum Chaperone BiP; Glucose-Regulated Protein, 78kDa; 78 KDa Glucose-Regulated Protein; Binding-Immunoglobulin Protein; HSP70 Family Protein 5; BiP; BIP; Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD); Endoplasmic Reticulum Lumenal Ca(2+)-Binding; Protein Grp78; Epididymis Secretory Sperm Binding Protein Li 89n; EC 3.6.4.10; HEL-S-89n; GRP-78
Background

Gene Name: HSPA5

NCBI Gene Entry: 3309

UniProt Entry: P11021
Application Information

Molecular Weight: Predicted, 72 kDa, observed, 74 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2445

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human GRP78 BiP
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of HSPA5 expression in HepG2 cells using HSPA5 antibody (Cat#61474, 1:2,000). Green, isotype control; red, HSPA5.
  • Immunocytochemical staining of HepG2 cells with anti-HSPA5 antibody (Cat#61474, 1:1,000). Nuclei were stained blue with DAPI; HSPA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-HSPA5 antibody (Cat#61474). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPA5 antibody (Cat#61474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). HSPA5, heat shock protein family A (Hsp70) member 5.
  • Western blotting analysis using anti-HSPA5 antibody (Cat#61474). HSPA5 expression in wild type (WT) and HSPA5 shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-HSPA5 antibody (Cat#61474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    HSPA5
  • 货号：
    61474
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of HSPA5 expression in HepG2 cells using HSPA5 antibody (Cat#61474, 1:2,000). Green, isotype control; red, HSPA5.
Immunocytochemical staining of HepG2 cells with anti-HSPA5 antibody (Cat#61474, 1:1,000). Nuclei were stained blue with DAPI; HSPA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-HSPA5 antibody (Cat#61474). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPA5 antibody (Cat#61474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). HSPA5, heat shock protein family A (Hsp70) member 5.
Western blotting analysis using anti-HSPA5 antibody (Cat#61474). HSPA5 expression in wild type (WT) and HSPA5 shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-HSPA5 antibody (Cat#61474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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