Flow cytometric analysis of Endothelin receptor type B expression in HepG2 cells using anti-Endothelin receptor type B antibody (Cat#61592, 1:2,000). Green, isotype control; red, Endothelin receptor type B.
Immunocytochemical staining of HepG2 cells with anti-Endothelin receptor type B1 antibody (Cat#61592, 1:1,000). Nuclei were stained blue with DAPI; Endothelin receptor type B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-endothelin receptor type B antibody (Cat#61592). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-endothelin receptor type B antibody (Cat#61592, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-endothelin receptor type B antibody (Cat#61592). Endothelin receptor type B expression in wild-type (WT) and endothelin receptor type B (EDNRB) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-endothelin receptor type B antibody (Cat#61592, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
