Flow cytometric analysis of Phospho-PKC alpha (T638) expression in HeLa cells using Phospho-PKC alpha (T638) antibody (Cat#61692, 1:2,000). Green, isotype control; red, Phospho-PKC alpha (T638).
Immunocytochemical staining of Hela cells with Phospho-PKC alpha (T638) antibody (Cat#61692, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PKC alpha (T638) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-PKC alpha (T638) antibody (Cat#61692). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PKC alpha (T638) antibody (Cat#61692, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-PKC alpha (T638) antibody (Cat#61692). Phospho-PKC alpha (T638) expression in wild type (WT) and Phospho-PKC alpha (T638) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-PKC alpha (T638) antibody (Cat#61692, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
