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KD-Validated Anti-IGF2R Rabbit Monoclonal Antibody #61979

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Western blotting analysis using anti-IGF2R antibody (Cat#61979). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IGF2R antibody (Cat#61979, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-IGF2R antibody (Cat#61979). IGF2R expression in wild-type (WT) and IGF2R shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IGF2R antibody (Cat#61979, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using anti-IGF2R antibody (Cat#61979, 1:1,000), Top panel: wild-type (WT); Bottom panal: IGF2R shRNA knockdown (KD). Nuclei were stained blue with DAPI; IGF2R was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名:
    IGF2R
  • 货号:
    61979
  • 应用:
    WB, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-IGF2R Rabbit Monoclonal Antibody
Aliases IGF2R; Insulin Like Growth Factor 2 Receptor; MPRI; CI-M6PR; CI-MPR; MPR300; CD222; CIMPR; M6P-R; MPR1; Cation-Independent Mannose-6-Phosphate Receptor; Insulin-Like Growth Factor II Receptor; Insulin-Like Growth Factor 2 Receptor; 300 KDa Mannose 6-Phosphate Receptor; CI Man-6-P Receptor; M6P/IGF2 Receptor; IGF-II Receptor; M6P/IGF2R; MPR 300; Cation-Independent Mannose-6 Phosphate Receptor; CD222 Antigen; M6PR
Background

Gene Name: IGF2R

NCBI Gene Entry: 3482

UniProt Entry: P11717

Application Information

Molecular Weight: Predicted, 274 kDa; observed, 300 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6790

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human M6PR/IGF2R
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:4,000-1:20,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-IGF2R antibody (Cat#61979). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IGF2R antibody (Cat#61979, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-IGF2R antibody (Cat#61979). IGF2R expression in wild-type (WT) and IGF2R shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IGF2R antibody (Cat#61979, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells using anti-IGF2R antibody (Cat#61979, 1:1,000), Top panel: wild-type (WT); Bottom panal: IGF2R shRNA knockdown (KD). Nuclei were stained blue with DAPI; IGF2R was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
Western blotting analysis using anti-IGF2R antibody (Cat#61979). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IGF2R antibody (Cat#61979, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-IGF2R antibody (Cat#61979). IGF2R expression in wild-type (WT) and IGF2R shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IGF2R antibody (Cat#61979, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using anti-IGF2R antibody (Cat#61979, 1:1,000), Top panel: wild-type (WT); Bottom panal: IGF2R shRNA knockdown (KD). Nuclei were stained blue with DAPI; IGF2R was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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