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KD-Validated Anti-Cyclin Dependent Kinase 2 Rabbit Monoclonal Antibody #63162

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Flow cytometric analysis of cyclin dependent kinase 2 expression in HepG2 cells using anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:2,000). Green, isotype control; red, cyclin dependent kinase 2.
Immunocytochemical staining of HepG2 cells with anti-Cyclin dependent kinase 2 antibody(Cat#63162, 1:1,000) . Nuclei were stained blue with DAPI; Cyclin dependent kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-cyclin dependent kinase 2 antibody (Cat#63162). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-cyclin dependent kinase 2 antibody (Cat#63162). Cyclin dependent kinase 2 expression in wild type (WT) and cyclin dependent kinase 2 (CDK2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Cyclin dependent kinase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cyclin dependent kinase 2 antibody (Cat#63162, 1:2,000) and analyzed using BD flow cytometer.
Immunohistochemistry was performed on paraffin-embedded human melanoma using anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名:
    CDK2
  • 货号:
    63162
  • 应用:
    WB, FCM, IC, IHC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Cyclin Dependent Kinase 2 Rabbit Monoclonal Antibody
Aliases CDK2; Cyclin Dependent Kinase 2; Cell Division Protein Kinase 2; Cyclin-Dependent Kinase 2; P33 Protein Kinase; EC 2.7.11.22; CDKN2; Cdc2-Related Protein Kinase; P33(CDK2); EC 2.7.11
Background

Gene Name: CDK2

NCBI Gene Entry: 1017

UniProt Entry: P24941

Application Information

Molecular Weight: Predicted, 34 kDa, observed, 34 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4315

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human Cdk2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Flow cytometric analysis of cyclin dependent kinase 2 expression in HepG2 cells using anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:2,000). Green, isotype control; red, cyclin dependent kinase 2.
  • Immunocytochemical staining of HepG2 cells with anti-Cyclin dependent kinase 2 antibody(Cat#63162, 1:1,000) . Nuclei were stained blue with DAPI; Cyclin dependent kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-cyclin dependent kinase 2 antibody (Cat#63162). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-cyclin dependent kinase 2 antibody (Cat#63162). Cyclin dependent kinase 2 expression in wild type (WT) and cyclin dependent kinase 2 (CDK2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of Cyclin dependent kinase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cyclin dependent kinase 2 antibody (Cat#63162, 1:2,000) and analyzed using BD flow cytometer.
  • Immunohistochemistry was performed on paraffin-embedded human melanoma using anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
Flow cytometric analysis of cyclin dependent kinase 2 expression in HepG2 cells using anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:2,000). Green, isotype control; red, cyclin dependent kinase 2.
Immunocytochemical staining of HepG2 cells with anti-Cyclin dependent kinase 2 antibody(Cat#63162, 1:1,000) . Nuclei were stained blue with DAPI; Cyclin dependent kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-cyclin dependent kinase 2 antibody (Cat#63162). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-cyclin dependent kinase 2 antibody (Cat#63162). Cyclin dependent kinase 2 expression in wild type (WT) and cyclin dependent kinase 2 (CDK2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Cyclin dependent kinase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cyclin dependent kinase 2 antibody (Cat#63162, 1:2,000) and analyzed using BD flow cytometer.
Immunohistochemistry was performed on paraffin-embedded human melanoma using anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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