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KD-Validated Anti-CRLF3 Mouse Monoclonal Antibody #63834

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Flow cytometric analysis of Cytokine receptor like factor 3 expression in HepG2 cells using anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:2,000). Green, isotype control; red, Cytokine receptor like factor 3.
Immunocytochemical staining of HepG2 cells with anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:500) . Nuclei were stained blue with DAPI; Cytokine receptor like factor 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-cytokine receptor like factor 3 antibody (Cat#63834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cytokine receptor like factor 3 antibody (Cat#63834, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cytokine receptor like factor 3 antibody (Cat#63834). Cytokine receptor like factor 3 expression in wild-type (WT) and cytokine receptor like factor 3 (CRLF3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cytokine receptor like factor 3 antibody (Cat#63834, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Cytokine receptor like factor 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cytokine receptor like factor 3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cytokine receptor like factor 3 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    CRLF3
  • 货号：
    63834
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-CRLF3 Mouse Monoclonal Antibody
Aliases CRLF3; Cytokine Receptor Like Factor 3; CREME9; CYTOR4; Cytokine Receptor-Like Factor 3; Type I Cytokine Receptor-Like Factor P48; Cytokine Receptor-Related Protein 4; Cytokine Receptor-Like Molecule 9; CREME-9; CRLM9; P48.2; FRWS; P48
Background

Gene Name: CRLF3

NCBI Gene Entry: 51379

UniProt Entry: Q8IUI8
Application Information

Molecular Weight: Predicted, 50 kDa; observed, 51 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8640

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human CRLF3
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Cytokine receptor like factor 3 expression in HepG2 cells using anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:2,000). Green, isotype control; red, Cytokine receptor like factor 3.
  • Immunocytochemical staining of HepG2 cells with anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:500) . Nuclei were stained blue with DAPI; Cytokine receptor like factor 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-cytokine receptor like factor 3 antibody (Cat#63834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cytokine receptor like factor 3 antibody (Cat#63834, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-cytokine receptor like factor 3 antibody (Cat#63834). Cytokine receptor like factor 3 expression in wild-type (WT) and cytokine receptor like factor 3 (CRLF3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cytokine receptor like factor 3 antibody (Cat#63834, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of Cytokine receptor like factor 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cytokine receptor like factor 3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cytokine receptor like factor 3 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    CRLF3
  • 货号：
    63834
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Cytokine receptor like factor 3 expression in HepG2 cells using anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:2,000). Green, isotype control; red, Cytokine receptor like factor 3.
Immunocytochemical staining of HepG2 cells with anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:500) . Nuclei were stained blue with DAPI; Cytokine receptor like factor 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-cytokine receptor like factor 3 antibody (Cat#63834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cytokine receptor like factor 3 antibody (Cat#63834, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cytokine receptor like factor 3 antibody (Cat#63834). Cytokine receptor like factor 3 expression in wild-type (WT) and cytokine receptor like factor 3 (CRLF3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cytokine receptor like factor 3 antibody (Cat#63834, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Cytokine receptor like factor 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Cytokine receptor like factor 3 antibody (Cat#63834, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cytokine receptor like factor 3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cytokine receptor like factor 3 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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