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KD-Validated Anti-PAIP1 Rabbit Monoclonal Antibody #65214

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Flow cytometric analysis of PAIP1 expression in HepG2 cells using anti-PAIP1 antibody (Cat#65214, 1:2,000). Green, isotype control; red, PAIP1.

Western blotting analysis using anti-PAIP1 antibody (Cat#65214). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAIP1 antibody (Cat#65214, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-PAIP1 antibody (Cat#65214). PAIP1 expression in wild-type (WT) and PAIP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PAIP1 antibody (Cat#65214, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Validation of PAIP1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PAIP1 antibody (Cat#65214, 1:2,000) and analyzed using BD flow cytometer.

基因名：

PAIP1
货号：

65214
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

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Information

Product Name	KD-Validated Anti-PAIP1 Rabbit Monoclonal Antibody
Aliases	PAIP1; Poly(A) Binding Protein Interacting Protein 1; PABP-Interacting Protein 1; Polyadenylate-Binding Protein-Interacting Protein 1; PAIP-1; Poly(A)-Binding Protein-Interacting Protein 1; PABC1-Interacting Protein 1
Background	Gene Name: PAIP1 NCBI Gene Entry: 10605 UniProt Entry: Q9H074
Application Information	Molecular Weight: Predicted, 54 kDa; observed, 45-60 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB195 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human PAIP1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PAIP1 expression in HepG2 cells using anti-PAIP1 antibody (Cat#65214, 1:2,000). Green, isotype control; red, PAIP1.
Western blotting analysis using anti-PAIP1 antibody (Cat#65214). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAIP1 antibody (Cat#65214, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PAIP1 antibody (Cat#65214). PAIP1 expression in wild-type (WT) and PAIP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PAIP1 antibody (Cat#65214, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of PAIP1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PAIP1 antibody (Cat#65214, 1:2,000) and analyzed using BD flow cytometer.

Citation(0)

基因名：

PAIP1
货号：

65214
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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