Flow cytometric analysis of NEDD9 expression in H9c2 cells using anti-NEDD9 antibody (Cat#65269, 1:2,000). Green, isotype control; red, NEDD9.
Western blotting analysis using anti-NEDD9 antibody (Cat#65269). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD9 antibody (Cat#65269, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NEDD9 antibody (Cat#65269). NEDD9 expression in wild-type (WT) and NEDD9 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NEDD9 antibody (Cat#65269, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human NEDD9
Isotype
Mouse IgG2a
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:2,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of NEDD9 expression in H9c2 cells using anti-NEDD9 antibody (Cat#65269, 1:2,000). Green, isotype control; red, NEDD9.
Western blotting analysis using anti-NEDD9 antibody (Cat#65269). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD9 antibody (Cat#65269, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NEDD9 antibody (Cat#65269). NEDD9 expression in wild-type (WT) and NEDD9 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NEDD9 antibody (Cat#65269, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NEDD9 expression in H9c2 cells using anti-NEDD9 antibody (Cat#65269, 1:2,000). Green, isotype control; red, NEDD9.
Western blotting analysis using anti-NEDD9 antibody (Cat#65269). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD9 antibody (Cat#65269, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NEDD9 antibody (Cat#65269). NEDD9 expression in wild-type (WT) and NEDD9 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NEDD9 antibody (Cat#65269, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
