KD-Validated Anti-PREB Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-PREB Rabbit Monoclonal Antibody #65556

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Flow cytometric analysis of PREB expression in HepG2 cells using anti-PREB antibody (Cat#65556, 1:2,000). Green, isotype control; red, PREB.

Western blotting analysis using anti-PREB antibody (Cat#65556). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PREB antibody (Cat#65556, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-PREB antibody (Cat#65556). PREB expression in wild-type (WT) and PREB shRNA knockdown (KD) HeLa cells with 25 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PREB antibody (Cat#65556, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

PREB
货号：

65556
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-PREB Rabbit Monoclonal Antibody
Aliases	PREB; Prolactin Regulatory Element Binding; SEC12; Prolactin Regulatory Element-Binding Protein; Guanine Nucleotide-Exchange Factor SEC12; Mammalian Guanine Nucleotide Exchange Factor MSec12
Background	Gene Name: PREB NCBI Gene Entry: 10113 UniProt Entry: Q9HCU5
Application Information	Molecular Weight: Predicted, 45 kDa; observed, 45 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB4240 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human PREB
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PREB expression in HepG2 cells using anti-PREB antibody (Cat#65556, 1:2,000). Green, isotype control; red, PREB.
Western blotting analysis using anti-PREB antibody (Cat#65556). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PREB antibody (Cat#65556, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PREB antibody (Cat#65556). PREB expression in wild-type (WT) and PREB shRNA knockdown (KD) HeLa cells with 25 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PREB antibody (Cat#65556, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

PREB
货号：

65556
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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