Flow cytometric analysis of Argonaute RISC catalytic component 2 expression in C2C12 cells using Argonaute RISC catalytic component 2 antibody (Cat#67352, 1:2,000). Green, isotype control; red, Argonaute RISC catalytic component 2.
Immunocytochemical staining of C2C12 cells with Argonaute RISC catalytic component 2 antibody (Cat#67352, 1:1,000). Nuclei were stained blue with DAPI; Argonaute RISC catalytic component 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Argonaute RISC catalytic component 2 antibody (Cat#67352). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Argonaute RISC catalytic component 2 antibody (Cat#67352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Argonaute RISC catalytic component 2 antibody (Cat#67352). Argonaute RISC catalytic component 2 expression in wild type (WT) and Argonaute RISC catalytic component 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Argonaute RISC catalytic component 2 antibody (Cat#67352, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-Argonaute RISC catalytic component 2 antibody (Cat#67352, 1:1,000), Top panel: wild-type (WT); Bottom panal: Argonaute RISC catalytic component 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Argonaute RISC catalytic component 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
