首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

  • 一抗
  • 二抗
  • 慢病毒
  • 细胞系
  • 裂解物
  • 试剂类
首页>产品中心>一抗

KO-Validated Anti-p53 (acetyl K370) Rabbit Monoclonal Antibody #71224

一键复制产品信息

返回列表

Flow cytometric analysis of p53 (acetyl K370) expression in HepG2 cells using anti-p53 (acetyl K370) antibody (Cat#71224, 1:2,000). Green, isotype control; red, p53 (acetyl K370).
Immunocytochemical staining of HepG2 cells with anti-p53 (acetyl K370) antibody (Cat#71224, 1:1,000). Nuclei were stained blue with DAPI; p53 (acetyl K370) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-p53 (acetyl K370) antibody (Cat#71224). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p53 (acetyl K370) antibody (Cat#71224, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-p53 (acetyl K370) antibody (Cat#71224). p53 (acetyl K370) expression in wild type (WT) and TP53 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-p53 (acetyl K370) antibody (Cat#71224, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    TP53
  • 货号：
    71224
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KO-Validated Anti-p53 (acetyl K370) Rabbit Monoclonal Antibody
Aliases TP53; Tumor Protein P53; P53; LFS1; Cellular Tumor Antigen P53; Phosphoprotein P53; Antigen NY-CO-13; Transformation-Related Protein 53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome; Tumor Suppressor P53; Tumor Supressor P53; Tumor Protein 53; BMFS5; TRP53; BCC7
Background

Gene Name: TP53

NCBI Gene Entry: 7157

UniProt Entry: P04637
Application Information

Molecular Weight: Predicted, 44 kDa; observed, 53 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3240

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human p53 (acetyl K370)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of p53 (acetyl K370) expression in HepG2 cells using anti-p53 (acetyl K370) antibody (Cat#71224, 1:2,000). Green, isotype control; red, p53 (acetyl K370).
  • Immunocytochemical staining of HepG2 cells with anti-p53 (acetyl K370) antibody (Cat#71224, 1:1,000). Nuclei were stained blue with DAPI; p53 (acetyl K370) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-p53 (acetyl K370) antibody (Cat#71224). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p53 (acetyl K370) antibody (Cat#71224, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-p53 (acetyl K370) antibody (Cat#71224). p53 (acetyl K370) expression in wild type (WT) and TP53 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-p53 (acetyl K370) antibody (Cat#71224, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    TP53
  • 货号：
    71224
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of p53 (acetyl K370) expression in HepG2 cells using anti-p53 (acetyl K370) antibody (Cat#71224, 1:2,000). Green, isotype control; red, p53 (acetyl K370).
Immunocytochemical staining of HepG2 cells with anti-p53 (acetyl K370) antibody (Cat#71224, 1:1,000). Nuclei were stained blue with DAPI; p53 (acetyl K370) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-p53 (acetyl K370) antibody (Cat#71224). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p53 (acetyl K370) antibody (Cat#71224, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-p53 (acetyl K370) antibody (Cat#71224). p53 (acetyl K370) expression in wild type (WT) and TP53 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-p53 (acetyl K370) antibody (Cat#71224, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 联系电话

    0551-63635518

    0551-65578998

  • 联系邮箱

    support@genuinbiotech.cn

    sales@genuinbiotech.cn

微信公众号

Copyright © 2025 合肥善本生物科技有限公司

All Rights Reserved. 皖ICP备2023000814号