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Human PRMT5 Knockdown Cell Line (Wb-Validated) #C62439

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RT-qPCR analysis. HeLa cells were infected with PRMT5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PRMT5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PRMT5 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    PRMT5
  • 货号:
    C62439
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human PRMT5 Knockdown Cell Line (Wb-Validated)
Aliases PRMT5; Protein Arginine Methyltransferase 5; SKB1Hs; HRMT1L5; SKB1; Histone-Arginine N-Methyltransferase PRMT5; Protein Arginine N-Methyltransferase 5; Shk1 Kinase-Binding Protein 1 Homolog; 72 KDa ICln-Binding Protein; Jak-Binding Protein 1; SKB1 Homolog; IBP72; JBP1; HMT1 HnRNP Methyltransferase-Like 5; Skb1 (S. Pombe) Homolog; SKB1 Homolog (S. Pombe); EC 2.1.1.320; HSL7
Background

Gene Name: PRMT5

NCBI Gene Entry: 10419

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PRMT5 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PRMT5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PRMT5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PRMT5 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with PRMT5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PRMT5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PRMT5 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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