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Human MMUT Knockdown Cell Line (Wb-Validated) #C61805

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RT-qPCR analysis. HeLa cells were infected with MMUT-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. MMUT protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against MMUT and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    MMUT
  • 货号：
    C61805
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human MMUT Knockdown Cell Line (Wb-Validated)
Aliases MMUT; Methylmalonyl-CoA Mutase; MCM; MUT; Methylmalonyl-CoA Mutase, Mitochondrial; Methylmalonyl Coenzyme A Mutase; Methylmalonyl-CoA Isomerase; EC 5.4.99.2; Methylmalonyl-CoA Mutase Variant C.613_615delGAA; Methylmalonyl-CoA Mutase Variant C.1495G>A; Methylmalonyl-CoA Mutase Variant C.2011A>G; Methylmalonyl-CoA Mutase Variant C.2150G>T; Methylmalonyl-CoA Mutase Variant C.322C>T; Methylmalonyl-CoA Mutase Variant C.636G>A; Methylmalonyl-CoA Mutase Variant C.643G>A; Methylmalonyl-CoA Mutase C.*192delA; Methylmalonyl-CoA Mutase C.*51C>G; Mutant Methylmalonyl CoA Mutase
Background

Gene Name: MMUT

NCBI Gene Entry: 4594
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human MMUT Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with MMUT-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. MMUT protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against MMUT and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    MMUT
  • 货号：
    C61805
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with MMUT-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. MMUT protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against MMUT and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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