Immunocytochemical analysis of Histone H2A.X (Phospho-S139) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Western blotting analysis of Histone H2A.X (Phospho-S139) expression in NIH3T3 treated with Hydroxyurea (A) whole cell lysates.
Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human Phospho-Histone H2A.X (S139)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.3) containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:1,000 Immunocytochemistry (IC): 1:50-1:100
Note
This product is for research use only.
Picture
Immunocytochemical analysis of Histone H2A.X (Phospho-S139) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Western blotting analysis of Histone H2A.X (Phospho-S139) expression in NIH3T3 treated with Hydroxyurea (A) whole cell lysates.
Immunocytochemical analysis of Histone H2A.X (Phospho-S139) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Western blotting analysis of Histone H2A.X (Phospho-S139) expression in NIH3T3 treated with Hydroxyurea (A) whole cell lysates.
