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Human PSMA1 Knockdown Cell Line (Wb-Validated) #C62604

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RT-qPCR analysis. HeLa cells were infected with PSMA1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PSMA1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PSMA1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    PSMA1
  • 货号：
    C62604
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

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Information
Product Name Human PSMA1 Knockdown Cell Line (Wb-Validated)
Aliases PSMA1; Proteasome 20S Subunit Alpha 1; PROS30; HC2; NU; Proteasome (Prosome, Macropain)Subunit,Alpha Type,1; Multicatalytic Endopeptidase Complex Subunit C2; Proteasome Subunit Alpha Type-1; Proteasome Subunit Alpha 1; 30 KDa Prosomal Protein; Proteasome Component C2;Macropain Subunit C2; Proteasome Nu Chain; MGC14542; MGC14575; MGC14751; MGC21459; MGC22853; MGC23915; MGC1667; PROS-30; Alpha-6; Epididymis Secretory Protein Li 275; Proteasome Subunit, Alpha-Type, 1; Testicular Tissue Protein Li 150; Proteasome Subunit Alpha 6; Proteasome Subunit Alpha-6; Proteasome Subunit Α6; Proteasome Subunit Nu; Macropain Subunit Nu; Protein P30-33K; EC 3.4.25.1; HEL-S-275; PSC2
Background

Gene Name: PSMA1

NCBI Gene Entry: 5682
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PSMA1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with PSMA1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PSMA1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PSMA1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    PSMA1
  • 货号：
    C62604
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with PSMA1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PSMA1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against PSMA1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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